Primary Productivity Protocol.

Abstract

PRE-CRUISE PREPARATIONS 1. Check well in advance of cruise to be sure there is enough isotope ready to be used. This amount may vary depending on specific activity desired. Also, check scintillation fluid and all other supplies on the supply list. 2. Be sure a copy of this protocol and a “Request for Isotope usage on SIO Vessels” (see sample forms section at the end of this Protocol) have been sent to the ship scheduling office. These items should be submitted three months in advance if possible. Submit these also for cruises NOAA vessel just as though it were an SIO vessel to extend licensing to the vessel. 3. Incubator tubes should be cleaned and calibrated prior to each cruise. After the Incubator tubes are calibrated, a new “desired depth” sheet must be prepared using the new calibration values. The new calibration values must also be entered into the PIC module of CODES for determining sampling depths at sea. 4. Polycarbonate bottles used for incubations should be cleaned with MICRO and acid rinsed prior to each cruise. 5. Rosette bottles used for Productivity sample collection must be MICRO cleaned and acid rinsed prior to each cruise. 6. Make a set of stick on labels scintiallation vials with sequential numbers for use on the cruise. Each CalCOFI productivity experiment uses 18 vials; typical cruises have 15 or 16 experiments.