EMP Protocols

 

Recent Submissions

  • EMP Sample Submission Guide. 

    Thompson, Luke; Ackermann, Gail; Humphrey, Greg; Gilbert, Jack; Jansson, Janet; Knight, Rob (NOAA Fisheries Southwest Fisheries Research Center, La Jolla,CA, 2018)
    This protocol was designed for collaborators with the Earth Microbiome Project to contribute samples in a standardized fashion. Raw, frozen samples are submitted in individually labeled tubes, 10 aliquots (identical ...

  • Earth Microbiome Project (EMP) high throughput (HTP) DNA extraction protocol. Version 07112018. 

    Marotz, Lisa; Schwartz, Tara; Thompson, Luke; Humphrey, Greg; Gogul, Grant; Gaffney, James; Amir, Amnon; Knigh, Rob (University of California, San Diego, San Diego, CA, 2018)
    This protocol accompanies the following publication: Marotz, Clarisse, et al. "DNA extraction for streamlined metagenomics of diverse environmental samples." BioTechniques 62.6 (2017):290-293

  • Quanti-iT™ Pico Green dsDNA Assay (Invitrogen P7589). 

    Poulos, Bonnie (Matthew Sullivan Lab, University of Arizona/Ohio State University, Tucson, AZ, 2019)
    This protocol accompanies the following publication: Ul-Hasan S, Bowers RM, Figueroa-Montiel A, Licea-Navarro AF, Beman JM, Woyke T, et al. (2019) Community ecology across bacteria, archaea and microbial eukaryotes in the ...